Our work describing a new crosslinking mass spectrometry technology for studying conformational and structural changes in proteins and proteins complexes has been published in eLife.
Jie Luo and Jeff Ranish collaborated on a project to determine the molecular architecture of the NuA4/Tip60 co-activator complex. This protein complex functions to allow proper control of gene expression and DNA repair by allowing other proteins to access the DNA in the cell nucleus. The new information about the structure and function of TIP60 could provide insight into different diseases where the protein complex plays a role, such as Alzheimer’s and various cancers. The work was published in Science.
Dynamic conformational and structural changes in proteins and protein complexes play a central and ubiquitous role in the regulation of protein function, yet it is very challenging to study these changes, especially for large protein complexes, under physiological conditions. To address this challenge, we created a novel isobaric crosslinker, Qlinker, for studying conformational and structural changes in proteins and protein complexes using quantitative crosslinking mass spectrometry (qCLMS).
Completing a years-long collaboration with the Taatjes and Kaplan labs, we publish an article in JBC that identifies key residues within the p52/Tfb2 subunit that control TFIIH function at transcription start sites in human and yeast cells.
In the cellular process of differentiation, information about the concentrations of an important class of proteins residing in a cell’s nucleus has been lacking, a missing link needed for scientists to fully understand how the process works. ISB researchers have quantified this important class of proteins that play a key role in the formation of red blood cells.
The Innovator Award Program at Institute for Systems Biology is an annual internal initiative started in 2017 that aims to stimulate creativity, innovation and collaboration within ISB, to provide funding support for high-risk, high-reward projects, and to develop new technologies and discoveries that will impact the entire research organization.
We are pleased to announce the promotion of Jeff Ranish to Professor. Jeff has been a pioneer in the fields of mass spectrometry and gene regulation. His impressive history of innovation in mass spectrometry technology has produced novel protein-crosslinking approaches to identify large scale protein-protein interactions in complex mixtures. He has applied the innovations to important biological problems, resulting in notable findings such as the characterization of multiple topological and…
Congratulations to Dr. Jie Luo, of the Ranish Lab, who has been promoted to senior research scientist. From Dr. Jeff Ranish: Jie Luo is an extremely dedicated and talented scientist. When he joined ISB in 2006 as a postdoctoral fellow, he had no experience in mass spectrometry-based proteomics. Now he is a leader in the field of structural and functional proteomics. He has conceptualized and implemented several innovative proteomics strategies…
General transcription factor TFIIH plays central roles in gene transcription and DNA repair ISB researchers and collaborators map the architecture of the TFIIH complex using powerful crosslinking-mass spectrometry (CXMS) technology and integrative modeling Structural maps provide critical insights into how mutations in TFIIH subunits lead to disease phenotypes By Jie Luo and Mark Gillespie The expression, or transcription, of genes controls the identity and function of a cell. DNA damage…
3 Bullets: Inflammation causes cholesterol buildup and leads to cardiovascular disease, the leading cause of death in the world ISB, Seattle Biomed, and Oregon State University researchers collaborate to identify a compendium of proteins that control expression of a key regulator of cholesterol efflux Targeting cholesterol efflux to HDL is a potentially important therapeutic strategy for preventing and treating cardiovascular disease By Dr. Mark Gillespie Cells of the immune system,…
3 Bullets: Aggressive tumor growth is linked to high activity of a macromolecular assembly called RNA polymerase I. ISB and FHCRC researchers collaborate to map the architecture of the assembly using a powerful crosslinking-mass spectrometry (CXMS) technology. Structural maps provide important insights into therapeutic targets for cancer treatment. By Mark Gillespie Rapidly growing tumor cells require large amounts of protein for their survival. This increased protein synthesis, or translation, can…
By Sui Huang and Joseph Zhou, ISB Editorial Board Members Cancer cells, for decades regarded as a uniform mass of identical (“clonal”) cells, are not like the soldiers of a traditional army, trained to act and respond in unison. Cancer cells, even within a genetic clone, express enormous individuality akin to guerrilla fighters, each with unique strengths, weaknesses and distinct behaviors. Therefore, they do not respond to an attack from…
Congratulations to Dr. Jeff Ranish on his promotion to Associate Professor. Jeff has been with ISB since its founding in 2000. Read about the Ranish lab group.
